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The membranes were blocked with 5% BSA and incubated with primary antibodies at 4 C for 12 h, followed by secondary antibodies (HRP-conjugated Goat Anti-Mouse IgG, Proteintech, SA00001-1, 1:20000) for 1 h at room temperature

Recordings were done on dissociated hippocampal neurons treated with vehicle, 10 "12 M Nlel -AnglV or Dihexa for 5 days prior to recording

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Muscles, tendons, ligaments, nerves, and connective tissue all depend on adequate nutrients to repair microdamage, regulate inflammation, and maintain structural integrity

Internalizing these principles is what elevates your research