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BBB dysfunction also increases with aging, which is an important risk factor for COVID severity 48,49

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siRNA silencing MODE-K IECs were seeded on 6-well plates at ~70% confluence for siRNA silencing with either Gpx4 siRNA ( siGpx4 , Ambion, s122098), Alox12 ( siAlox12 , Ambion, s62261), Alox15 ( siAlox15 , Ambion, s62271) or scrambled control siRNA (siCtrl, Ambion 4390843) and RNAiMAX (Thermo Fisher Scientific, 13778100) transfection over 48 h unless otherwise indicated, according to the recommended protocol

Determination of the nuclear maturation stage After maturation, the oocytes were washed in PBS-PVA, denuded from cumulus cells by gentle pipetting, fixed in 0.5% glutaraldehyde (v/v) for 15 min and stored at 4 C