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To mimic smoking, mice were treated with cigarette smoke extract or with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a powerful AhR ligand

TSPO radioligand binding correlates with the presence of activated microglia identified by immunohistochemistry in situ [12] and immunohistochemistry combined with autoradiography in brain tissue [9, 13], and is elevated in illnesses known to be associated with gliosis [14], though TSPO signal also arises from astroglia and endothelial cells [15]

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[10] Thom Bresh, the son of the song's writer Merle Travis, hit #78 on the Country Charts with the song in 1978