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Cells were lysed using RIPA buffer (50 mM Tris pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, and 0.1% sodium dodecyl sulfate, 2 mM DTT, 2 mM sodium orthovanadate, 1 protease inhibitor cocktail [Roche])

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In this special episode of Joint Action, Professor David Hunter reflects on the proposed changes to research funding and collaboration threaten global science and why speaking up, supporting one another, and protecting open, rigorous research matters now more than ever.PROVIDE FEEDBACKOffice of Management and Budget (Open until July 13th, 2026) - Federal Register :: Regulation for Federal Financial AssistanceCONNECT WITH USNaia Health: one of our trials @ProfDavidHunterTwitter: @ProfDavidHunter @jointactionorgEmail: [email protected]: www.jointaction.info/podcast 12

10.1016/j.jaad.2016.02.1228 26 CallouT