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Interestingly, it was recently found that chronic shift lag induces NK cells aging and impairs immunosurveillance 99

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Then, 100 L of this combination was evenly spread onto microscope slides, which were immersed in a lysis solution made of NaCl (2.5 M), EDTA (100 mM), TRIS buffer (10 mM), 1% lauryl sarcosinate, 1% Triton X-100, and 10% DMSO for 15 min