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4,373,539 (12) describes a smoking device comprising a means to hold a coiled helical tube filled with compressed carbon or activated charcoal having an inside diameter of approximately one-eight inch ( 3.125 mm) and a length of approximately one and one-quarter inches ( 31.25 mm) which when uncoiled is approximately six inches ( 150 mm) long

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The incubation system contained 1 mg BSA and 50 l of smoke solution obtained from cigarettes with or without charcoal filter in a final volume of 200 l of 50 mM potassium phosphate buffer, pH 7.4