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Finally, to establish the effect of water on volatiles profile, a representative product (mango-flavored hookah tobacco) was analyzed in the presence of water at 190 C and results of attained volatiles were compared to previous dry analysis (Supplementary Fig

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68 No toxicity, mutagenicity, or immunotoxicity were documented for mice inhaling a tobacco substitute primarily composed of Eucommia ulmoides leaf at levels of up to 20 cigarettes per day over 4 weeks