To investigate the molecular mechanisms underlying curcumin's antimetastatic activity, one study employed qPCR and Western blotting to detect changes in gene and protein expression ( in vitro and in vivo models ( Another study also investigated the role of the p53/miR-34 axis in mediating the anticancer effects of curcumin in CRC ( In vivo , intravenous injection of CRC cells into non-obese diabetic/severe combined immunodeficiency (NOD/SCID) mice showed that curcumin prevented lung metastasis formation in a miR-34a-dependent manner, suggesting curcumin induces mesenchymal-to-epithelial transition (MET) ( 3.1.5 Reversal of chemoresistance In another study, the effects of curcuminoids on CRC were investigated using two human colon cancer cell lines, human tumor 29 (HT29) and SW480, alongside normal CCD-18Coa human colon fibroblast cell line derived from normal colon tissue ( In another study using HCT116 and SW620 colon cancer cell lines, researchers investigated the molecular mechanism underlying curcumin's anticancer activity, with a particular focus on the miR-134-5p/CDCA3/CDK1 signaling pathway ( in vivo , SW620 cells were injected into mice to establish xenograft tumors, allowing for evaluation of curcumin's effects on tumor progression ( In vivo experiments supported these findings, as curcumin treatment significantly reduced tumor growth in mice, accompanied by increased miR-134-5p expression and reduced CDCA3 and CDK1 levels
The moderately efficient enzyme: evolutionary and physicochemical trends shaping enzyme parameters
A 1-year controlled trial of acetyl-l-carnitine in early onset AD
References World Malaria Report (WHO, 2023)
10.1038/s41598-017-14072-x 14 ColeA
