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Very low levels of these enzymes have also been detected in the extracellular cell wall/apoplastic space (Vanacker et al

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Biotinylated monomers were dialyzed against an NaCl 150 mM, Tris 10 mM pH 8.0 buffer and purified on a monomeric streptavidin affinity column (Thermofisher) by elution with D-biotin

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