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Amplified fragments were purified with AMPure XP Beads (Beckmann Coulter Genomics), combined and analyzed by 2 300 bp paired-end sequencing using an Illumina MiSeq device

BPC 157 activated the Src-Cav-1-eNOS signal pathway and reduced eNOS/Cav-1 binding in vascular endothelial cells It is known that phosphorylation of Src can promote Cav-1 activation and subsequent endocytosis of tyrosine kinase receptors and eNOS activation 20,24

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Mitochondrial GR is essential for: Protecting the electron transport chain components from oxidative damage Maintaining mitochondrial DNA integrity against oxidative lesions Supporting ATP production by preserving mitochondrial membrane potential Preventing mitochondrial-initiated apoptosis triggered by excessive oxidative stress Facilitating mitochondrial glutathione transport and utilization The mitochondrial glutathione system is particularly critical in neurons and other cells with high energy demands, where mitochondrial dysfunction can have catastrophic consequences

The two are structurally unrelated and act through different biological pathways, which is the main reason researchers became interested in studying them in combination