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mRNA transcripts were quantified using LightCycler 480 SYBR Green I Master kit (Roche Diagnostics Ltd., UK) using a LightCycler 96 instrument
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Analysis of Central Nervous System Karyopyknotic Cells Modified Bielschowskys silver staining and KlverBarrera staining (using KlverBarrera Luxol fast blue) were performed to demonstrate argentophilic neurites, axonal spheroids, and neuronal cell bodies, particularly in brain karyopyknotic areas ( (file:/ The brain was dissected according to NTP-7 at Levels 3 and 6 with neuroanatomic subsites presented in certain brain sections using coronal sections with three mandatory sections (Eustis et al., 2017
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