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Rodents fed with HFD develop increased ovarian lipid accumulation, disrupted mitochondrial function, elevated ROS production, and chronic metabolic inflammation, closely mimicking PCOS and metabolic-related reproductive impairment (Gonnella et al., 2022)

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Although energy metabolism status can also regulate intracellular ROS levels (e.g., glucose deprivation leads to a marked increase in cellular ROS) [53,54,55,56], our study demonstrated that supplementation with energy substrates such as sodium pyruvate in GGCT-knockdown cell models did not induce significant alterations in intracellular ROS levels