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Both need to be monitored

Excessive peritoneal iron accumulation leads to overproduction of reactive oxygen species (ROS)-a group of highly reactive chemicals formed from oxygen, which at high concentrations can damage DNA, RNA, proteins, and lipids within the cell (20)-and enhanced activation of nuclear factor kappa-B (NF-B), which, by promoting the expression of matrix metalloproteinases (MMPs), exacerbates inflammation, angiogenesis, and cell adhesion

10.1016/j.bios.2022.114401

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Cells seeded in six-well plate were incubated with C11-BODIPY 581/591 at a final concentration of 10 M for 30 min at 37 C and washed three times with PBS