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12 weeks (2 vials): 6 mL 1 10 mL bottle 24 weeks (7 vials): 21 mL 3 10 mL bottles 48 weeks (17 vials): 51 mL 6 10 mL bottles Alcohol Swabs One for the vial stopper + one for the injection site each week

Overall, this study demonstrates that EsCu@NPs enable coordinated cuproptosis and immune reprogramming, expanding ICB efficacy and supporting a translatable neoadjuvant strategy in TNBC