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Western blot analysis and immunoprecipitation Total cells lysates for immunoblotting or immunoprecipitation were lysed with buffer (0.1%Triton-100, 50 mM Tris-Cl, pH 7.5, 0.1% NP-40, 150 mM NaCl, 0.1 M EDTA) supplemented with a cocktail of phosphate and proteinase inhibitors for 30 min at 4 C

This disease has major autoimmune character, with T-cells and other immune effector populations entering the brain and attacking the nerve cells, stripping away their myelin insulation and sometimes destroying their axons and entire remaining structures

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Aug 2014;8(8):e3019

All authors made substantial, direct and intellectual contribution to the work, and revised the manuscript