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The second one is oxidative degradation during heat treatment

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At the highest concentration of 5800 M, both CuCl 2 and Cu(OAc) 2 were found to be very cytotoxic to the cells and almost all the cells were dead at the three time points

The combination requires proper handling procedures consistent with growth hormone secretagogue research standards to maintain molecular integrity and selectivity throughout experimental protocols

Immunoprecipitation and immunoblotting Cells were initially lysed on a rotation wheel in ice-cold RIPA buffer (50 mM Tris, 150 mM NaCl, pH 8.0, 0.5% deoxycholate, 1% NP-40, 0.1% SDS) for 1 h