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Indeed translocation of mTORC1 from the cytoplasm to the lysosome, a rich compartment in amino acids, is critical for mTORC1 activation (21)

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Mice were then transcardially perfused with ice-cold PBS (Biological Industries) before spleens, deep cervical lymph nodes, Peyers patches, and mesenteric lymph nodes were excised and mashed with the plunger of a syringe against a 100 m pore size cell strainer (Corning)

Elevated cytosolic Ca 2+ levels enhance the Ca 2+ /CaM stress response signal, triggering the expression of stress-responsive genes and enzymes in stress-related metabolic pathways