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Therefore, metabolomics, which is the large-scale study of small molecule metabolic products (the metabolome) of cells, tissues, biofluids and organisms at a specific point in time

First, the starting dose should reduce the risk of toxicity

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Dimethyl sulfoxide diluted in a .01 M glycine buffer was added to solubilize the cell membranes and the absorbance of reduced MTT in the buffer was quantitated at 590 nm using a plate reader (Biotek Synergy 2, Winooski, VT)

MUC5AC protein expression was quantified by ELISA (Novus Biologicals, NBP2-76703) and LDH by a Lactate Dehydrogenase Assay Kit (Abnova, KA0878) according to the manufacturers instructions