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Experiments were performed at room temperature with glass pipettes (4-5M resistance) filled with the intracellular solution comprising 140 mM CsMeSO 3 , 4 mM NaCl, 1 mM MgCl 2 , 1 mM EGTA, 5 mM HEPES, 2 mM MgATP, and 0.6 mM NaGTP, pH = 7.4 (CsOH)

Its important to follow the right protocols

or 1/20, CHII -M

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