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1974 May;104(5):580-7

Leu can bind to Sestrin2 (a negative regulator of mTORC1 activity) to promote mTORC1 activation, 46 thereby promoting protein synthesis in the liver and other tissues

Separation was achieved on a Waters Premier BEH Z-HILIC column (1.7 m, 2.1 150 mm) using 20 mM ammonium bicarbonate solution with 0.1% ammonium hydroxide and 0.1% InfinityLab deactivator as mobile phase A and H 2 O/ACN (1:9 ratio) with 0.1% InfinityLab deactivator as mobile phase B