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Mechanisms of ephrin-Eph signalling in development, physiology and disease

Cell pellets were resuspended in 0.3 ml small-scale lysis buffer (20 mM potassium phosphate, 200 mM NaCl, pH 7.4, 1 mg ml 1 lysozyme, 0.75 mg ml 1 polymyxin B, 0.01 mg ml 1 DNase I) per well and incubated at 30 C, 300 rpm, 5 cm shaking diameter for 30 min

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While its diuretic effect was less pronounced than that of standard furosemide, it still showed significant potential in enhancing urine excretion and maintaining electrolyte balance [69]

Molecules 24 Dennis G Jr, Sherman BT, Hosack DA et al (2003) DAVID: Database for Annotation, Visualization, and Integrated Discovery