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Cells were prepared and stained as per the Leica protocol ( In brief, cells were plated onto coverslips and treated with or without 5FU at the indicated doses for 72 h

If possible, continue your protocol with semaglutide from a verified source to avoid abrupt discontinuation

FIGURE 2 We used the comparative delta-Ct method to ensure that GAPDH expression is consistent throughout the samples and groups

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