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Other studies have shown similar findings

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Nicotine even contaminates dust

44 Initial screenings of the inhibition potential of CBD and 7-OH-CBD against UGTs 1A4, 1A9, 2B7, 2B10, and 2B17 were performed similarly to that described above for CYP/FMO assays, using microsomes from UGT-overexpressing HEK293 cell lines or HLM (50100 g for both) in reactions containing either 10 or 100 M CBD or 7-OH-CBD, substrate at concentrations approaching their known K M s for a given enzyme (5 mM nicotine, 5 mM cotinine, or 30 mM 3HC 22,23,45 ), 50 mM TrisHCl buffer (pH 7.4), and 5 mM MgCl 2 in a final reaction volume of 25 L