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Primary and secondary sequences of every sample were analyzed using the Mutation surveyor (Version 5.2.0), BioEdit sequence alignment editor version 7.2.5, and Unipro UGENE (Version 50.0) software

(Two that appear to have been studiously avoided: Jarmusch once played a coffee addict in Alex Coxs 1987 comic western Straight to Hell , and in Wayne Wang and Paul Austers 1995 Blue in the Face he ruminated on what he claimed would be his last cigarette.) Jarmusch has noted in an interview that Tom Waitss speech to Iggy Pop in the third episodeabout having just performed roadside surgery by delivering a baby and about combining music and medicine in his lifewas improvised

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