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Western Blotting Purified protein samples were suspended in 1 sodium dodecyl sulfate (SDS) loading buffer (250 mM Tris-HCl pH 6.8, 40% glycerol (v/v), 10% SDS (w/v) (SB0485, Biobasic), 0.05% bromophenol blue (w/v) (0449-25G, Amresco), 5% 2-mercaptoethanol (M6250-100ML, Sigma)) and heated for 5 min at 95C

Interestingly, increased levels of exhaled NO can be associated with a higher risk of asthma (106), and can point to the coexistence of asthma in patients with chronic obstructive pulmonary disease (COPD) (112)

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ALA has been shown to increase cellular levels of glutathione and protect against oxidative stress