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The challenge, of course, is finding the time

Briefly, 400 ]x slices were cultured on (Miiipore, Billerica, MA) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gen Gun (BioRad, Hercules, CA), according to the manufacturer's protocol, to visualize dendritic arbors

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This aberrant activation of PARPs represses the SIRT1 activity and mitochondrial dysfunction, which can be rescued by both PARP inhibitor and NAD + precursors

as sex was a significant factor (3-way ANOVA, Supplementary Data 2) in the response of these outcomes and many other during the study, we analyzed the sexes separately