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Effect of fresh CB extract on larval mortality The effect of fresh CB microcosm also showed significant effects on larval mortality for all larval instars, i.e., 1 st ( F (3) = 273, P = 0.0000), 2nd ( F (3) = 47.4, P = 0.0000), 3rd ( F (3) = 83.2, P = 0.0000), and 4 th ( F (3) = 59.8, P = 0.0000) as shown in Fig