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Facial images were captured using the VISIA-CR imaging system (Canfield Scientific, USA)

Coefficient of Variance Intra Variation% 10% Inter Variation% 12% Recovery 95-102% Linearity Diluent Ratio Range % 1:2 93-105 1:4 88-106 1:8 86-108 Specificity/Cross-reactivity No significant cross-reactivity or interference between GSSG and analogues was observed

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The stained sections were subsequently dehydrated through an ethanol series, cleared with xylene, and mounted using a resinous medium for microscopic analysis

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