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Despite the added advantages of the freeze-drying method, there are a few disadvantages associated with it such as membrane integrity may be lost, encapsulated substances might leak out from the vesicle and vesicle size might decrease [163]

Deficiency of intracellular glutathione can worsen oxidative stress and accelerate cellular death in diseases such as Alzheimer disease, Parkinson disease, liver disease, cystic fibrosis, sickle cell anemia, HIV/AIDS and diabetes

Although these flexibilities are a conserved characteristic, these regions do not have conserved sequences (Fig 3)

CSL, cefoperazone-sulbactam

This process yielded a total of 7 conformations per trajectory