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Even the parent protein (TB4) failed to make it through the approval process

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We hypothesize that the failure of intrinsic-solubility approaches arises from inaccuracies in the predicted valuesalthough Starling is a state-of-the-art p K a -prediction model, it still routinely predicts values that are wrong by 0.51.0 p K a unit, which can correspond to a 10x difference in the predicted value