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Accurate elicitation of history and clinical signs is vital in selecting the correct confirmatory investigation

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The proteins were visualized by exposure machine (GE, Amersham Imager 680) using Pierce ECL Plus Western Blotting Substrate (Thermo Fisher Scientific, 32132) and the protein bands were quantitatively analyzed with ImageJ software

This is a lyophilized powder that typically requires reconstitution with a suitable sterile diluent before use, and careful handling to reduce contamination