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Zonal organization: Articular cartilage has superficial zone (tangential collagen orientation, highest cell density), middle zone (random collagen orientation, largest proteoglycans), deep zone (perpendicular collagen orientation, calcified interface with bone), and tidemark (boundary between calcified and uncalcified cartilage)
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Being off by even 0.2 mL changes your concentration enough to affect dosing accuracy over the life of the vial
However, cycling prevents potential receptor desensitization, allows integration of natural healing, and reflects appropriate caution given limited long-term human data
As noted above, the mid-region residues (S19 Cagri- P25 Cagri ) form the prototypical bypass motif that was observed for rAmy/San385, constrained by conserved intramolecular hydrogen bonds between the backbone of L16 Cagri /F23 Cagri and S20 Cagri , and the backbone of P25 Cagri and N21 Cagri (Fig
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