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[DOI] [PubMed] [Google Scholar] 44.Michieli P, Mazzone M, Basilico C, Cavassa S, Sottile A, Naldini L, et al

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BBB breakdown and dysfunction in cerebrovascular diseases lead to leakage of blood components into the brain parenchyma, contributing to neurological deficits

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In the first protocol, growth factor-reduced matrigel was pipetted into prechilled 24-well plates (150 mL matrigel per well) and polymerized for 45 minutes at 37C