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Therefore, noting the lack of spontaneous oxidation of free GSH between the two EDTA-treated samples stored at 80 o C, this result also suggests that the differences in GSH between the heparinized plasma (Preparation Condition 1) and deproteinized EDTA-treated plasma (Preparation Condition 2) are due to spontaneous oxidation of free GSH occurring in the heparinized plasma prior to the deproteinization process

Blank controls were set, in which DCFH-DA incubation was omitted

Storage: Store at controlled room temperature (20C to 25C)

Mice from both genders were equally included in the experimental paradigm