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Systems were equilibrated in isothermal-isobaric conditions (NPT) using the Berendsen barostat 51 with a target pressure of 1 atm, the Langevin thermostat 52 with a target temperature of 310 K, along with a low damping factor of 1 ps 1 and an integration time step of 2 fs

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Under heat stress stimulation in both in vivo mouse models and in vitro cell experiments, the transport of p53 protein from the nucleus to the cytoplasm increases significantly, where it binds to Parkin protein, reducing Parkin's translocation from the cytoplasm to mitochondria