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Following electrophoresis, proteins were transferred onto a PVDF membrane (Millipore) using a Bio-Rad Trans-Blot apparatus at 25 volts for 30 min

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The attachment coordinates of these ligands may use variously one, two or even all three donor atoms, which would then determine the ligands description as monodentate, bidentate or multidentate

4244 Hence, standardization of PRP preparation and minimization of heterogeneity are crucial to drawing credible conclusions

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