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Following treatment, the medium containing dead cells was removed, and the remaining cells were detached using trypsin, collected, and washed twice with Hanks Balanced Salt Solution (HBSS)

In the proximal small intestine, luminal absorption of low Ca 2+ concentrations involve mainly the transcellular route (vitamin D-dependent) and for high concentrations, the paracellular passive route (vitamin D-independent) is the predominant source for calcium absorption