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It should be kept away from strong chelators (such as high levels of EDTA and related sequestrants), reducing sugars, thiol-bearing actives, and high concentrations of direct acids (for example ascorbic acid or alpha-hydroxy acids at low pH), all of which can compete for the copper center or disrupt the peptide-metal bond and shift the characteristic blue color toward brown or colorless degradation products

However, it is unclear why genistein upregulates the levels of Nrf2 in the myocardium

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A clinical trial of the ASO tofersen in 50 patients with SOD1-ALS showed evidence of target engagement

These were cloned into the pET28a(+) expression vector (Novagen) using the NdeI and BamHI restriction sites and sequence verified