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Western blot analysis involved preparing whole cell lysates using RIPA lysis buffer, quantifying protein concentration via BCA assay, followed by SDS-PAGE, transfer to nitrocellulose membranes, incubation with primary and HRP-conjugated secondary antibodies, and detection using an ECL solution [1]

A positive relationship between protein synthetic rate and intracellular glutamine concentration in perfused rat skeletal muscle

SLC7A11 is a transmembrane protein with 12 transmembrane domains, with both its N- and C-termini located intracellularly ( Figure 2 )

As the preferred choice of vascular access for hemodialysis, AVF failure remains a significant barrier to successful fistula use

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