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It immediately supplies cysteine for repletion of glutathione and thus, recovery from toxicity

Studies in fly models of PD treated with NAM, NAD + , or through PARP mutations demonstrated increased NAD + levels, which also rescued mitochondrial function 283,284,285

After that 20 l of 1250 U/ml mushroom tyrosinase solution was added into each well to a final volume 250 l and incubated at 25C for another 5 minutes