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TMAO and endoplasmic reticulum kinase PERK TMAO, a gut microbiota-derived metabolite, has emerged as a critical modulator of ERS through its interaction with the PERK pathway

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Cells were homogenized and lysed with RIPA lysis buffer (100 mM NaCl, 50 mM Tris-HCl pH 7.5, 1% TritonX-100, 1 mM EDTA, 10 mM -glycerophosphate, 2 mM sodium vanadate and protease inhibitor)