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Expired BAC water: The benzyl alcohol breaks down over time, so the antibacterial protection drops off

Room temperature storage (2025C) accelerates benzyl alcohol degradation by approximately 100% compared to refrigerated storage reducing reliable use period from 28 days to 710 days for opened vials

Periportal assembloids To generate liver periportal assembloids comprising hepatocytes, cholangiocytes and portal fibroblasts, we first prepared the cellular components as follows: nGFP-labelled cholangiocyte organoids (passage 511), grown in cholangiocyte expansion medium (h-CholOrg-EM) as detailed above, were collected from Matrigel using cold AdDMEM/F12 (Invitrogen, 12634010) containing 1% HEPES (ThermoFisher, 15630-056), 1% penicillin/streptomycin (ThermoFisher, 15140-122) and 1% GlutaMAX (ThermoFisher, 35050038)

Please see the fact sheet for additional important information about storage and handling