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An intact solution appears clear blue from the copper complex

Each micrograph was dose-fractionated to 30 frames under a dose rate of 18 e /pixel/s, with a total exposure time of 4 s, resulting in a total dose of about 60 e / 2

the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1:5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for one hour at room temperature

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