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[20] The degree of enzyme thermolability (assessed as residual activity after heat inactivation) is much greater in 677TT individuals (1822%) compared with 677CT (56%) and 677CC (6667%)

After selection of 3 g/ml puromycin(Beyotime, Cat# ST551), TPC-1 and IHH4 cells were harvested for western blotting to assess RRM2 knockdown efficiency

Animal studies have not reported major toxicity, but the lack of comprehensive human data means risks are unknown467

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