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The lipid peaks were identified, aligned, and exported using MS-DIAL 98

The media was then removed and the cells were washed once with PBS, then incubated in PBS containing 1 M NAO and 1 M TMRE for 30 minutes at 37C

Effect of dietary garlic and onion on biliary proteins and lipid peroxidation which influence cholesterol nucleation in bile

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The cumulative effect builds over 2 to 4 weeks of consistent injections, which is why Triny typically recommends a loading phase before transitioning to monthly maintenance