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After removing the spent medium, cells were incubated in 500 l complete MEM containing antibiotics (10 g ml 1 Rif, 50 g ml 1 Mox or 20 g ml 1 Van) with or without 0100 M KL1 or its analogues (KL27), 20 M BHA (Sigma) or 010 M BIX-01294, in the presence of 50100 g ml 1 Gen at 37 C for 624 h

The pH is 5.7 (4.5 to 7.0)

The FDA can take it or leave it," Tatem noted

Its ability to interact with lipolytic pathways without the confounding growth-promoting effects of full GH makes it particularly useful for dissecting the metabolic arm of GH signalling in laboratory models