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FreSHtracer consists of a coumarin derivative bearing a conjugated 2-cyanoacrylamide group that reacts reversibly with GSH in aqueous solutions [19], leading to a spectral shift in the maximum wavelength ( max ) of ultravioletvisible absorption from 520 to 430 nm

Vitamin B12 deficiency can occur due to dietary restrictions (vegetarian or vegan diets), absorption issues (pernicious anemia or gut disorders like celiac disease or Crohns disease), age-related changes in elderly patients, or long-term use of certain medicines