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Hippocampal Neuron-Enriched Cultures Primary neuronal cultures were established from 18-day-old embryonic rat hippocampi, as previously described ( 2 ) glass coverslips, previously coated with poly(D-lysine) (50 g/ml) and then with DMEM/HAM F12 containing 10% FCS

Free Radic Biol Med 19:227250 Rapola JM, Virtamo J, Ripatti S, Huttunen JK, Albanes D, Taylor PR, Heinonen OP (1997) Randomised trial of alpha-tocopherol and beta-carotene supplements on incidence of major coronary events in men with previous myocardial infarction

Upon degranulation, they release histamine, stimulating the nerve to release substance P and glutamate from the nerve terminals

The initial treatment involves daily intramuscular (into the muscle) vitamin B12 injections

For example, you need to produce data on dosage, route of administration, and more