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The TNF- levels were determined using the rat TNF- ELISA kit from Boster Biological Technology (CA, United States), while IL-1 and IL-6 levels were measured using the rat IL-1 beta/IL-1F2 Quantikine ELISA Kit and the rat IL-6 Quantikine ELISA Kit from R&D Systems (MN, United States), respectively

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Nevertheless, targeting the Wnt pathway alone presents significant challenges for cancer therapy, including poor drug response and toxic side effects (100)

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doi:10.1038/s41419-021-03998-w