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ScienCell Research Laboratories, Carlsbad, CA] were cultured in their respective vendor suggested media

Polymorphisms in glutathione S-transferase are risk factors for perioperative acute myocardial infarction after cardiac surgery: A preliminary study

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Then, every total RNA sample was diluted to 1 ug and used to run one-step real-time PCR using Luna Universal Probe One-Step RT-qPCR Kit (New England BioLabs, Ipswich, MA, USA) with CFX96 System (Bio-rad, Hercules, CA, USA)